TOLERANCE INDUCTION BY THYMIC MEDULLARY EPITHELIUM
- Author(s)
- Hoffmann, MW; Allison, J; Miller, JFAP;
- Details
- Publication Year 1992-04-01,Volume 89,Issue #7,Page 2526-2530
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- To study the role of thymic medullary epithelium in tolerance induction, the third and fourth branchial clefts of embryos from E-mu-K(b) transgenic mice, which express the major histocompatibility complex class I antigen H-2K(b) exclusively on medullary thymic epithelium, were grafted to athymic nude mice. The grafts differentiated into tissue that morphologically resembled normal thymus. These grafts expressed the H-2K(b) antigen appropriately and gave rise to a functional T cell repertoire. In vivo tolerance to H-2K(b) disparate skin grafts was invariably found in mice expressing H-2K(b) in the medulla or in both medulla and cortex of C57BL/6 branchial cleft-grafted controls. In marked contrast, in vitro cytotoxicity assays demonstrated reactivity toward H-2K(b) in the presence of interleukin 2, and limiting-dilution analyses showed similar frequencies of cytolytic T cell precursors reactive to H-2K(b) and to third-party stimulators. Medullary epithelium can, therefore, induce split tolerance, in which in vivo tolerance is accompanied by strong in vitro responses in the presence of interleukin 2.
- Publisher
- NATL ACAD PRESS
- Keywords
- CLONAL DELETION; HISTOCOMPATIBILITY ANTIGENS; MONOCLONAL-ANTIBODIES; TRANSGENIC MICE; NUDE-MICE; T-CELLS; MOUSE; PRECURSORS; SUBSETS; ANERGY
- Publisher's Version
- https://doi.org/10.1073/pnas.89.7.2526
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-04-01 12:00:00