PRESENCE OF SINGLE-STRANDED-DNA IN PCR PRODUCTS OF SLOW ELECTROPHORETIC MOBILITY
- Author(s)
- VALENTINE, JE; Boyle, MJ; SEWELL, WA; Gough, NM;
- Details
- Publication Year 1992-08,Volume 13,Issue #2,Page 222-&
- Journal Title
- BIOTECHNIQUES
- Publication Type
- Journal Article
- Abstract
- PCR products were characterized by electrophoresis, blotting and hybridization. In addition to the bands of expected size, bands of slower electrophoretic mobility were often detected. The slower bands completely disappeared when the PCR products were subjected to slow cooling, treated with S1 nuclease or run on an alkaline gel, whereas the bands of expected size were unaffected. The slower bands are therefore likely to contain single-stranded DNA.
- Publisher
- EATON PUBLISHING CO
- Keywords
- EXPRESSION; POLYMERASE; GENE
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-08-01 12:00:00