AN ELISA FOR ANTIBODIES TO RECOMBINANT GLUTAMIC-ACID DECARBOXYLASE IN IDDM
- Author(s)
- DeAizpurua, HJ; Harrison, LC; Cram, DS;
- Details
- Publication Year 1992-09,Volume 41,Issue #9,Page 1182-1187
- Journal Title
- DIABETES
- Publication Type
- Journal Article
- Abstract
- To detect serum antibodies to GAD in subjects with IDDM, three recombinant mBGAD 67 peptides encompassing the full-length protein were used in an ELISA. In this study 7 of 9 (78%) preclinical IDDM subjects (ICA+ first-degree relatives of a person with IDDM) and 6 of 13 (46%) recent-onset IDDM subjects, but no subjects with Graves' disease (n = 10) or scleroderma (n = 10), nor healthy nondiabetic control subjects (n = 10) had antibodies that reacted with one or more of the recombinant mBGAD peptides. We found no preferential reactivity with any recombinant peptide. Although only 3 preclinical subjects and 1 recent-onset subject had antibodies to all three mBGAD peptides, the results indicate that mBGAD 67 contains at least three B-cell autoepitopes. Compared with an immunoprecipitation assay of native human brain GAD, the ELISA detected 5 of 6 (83%) preclinical and 6 of 6 (100%) recent-onset IDDM subjects. The ELISA should facilitate screening to evaluate the role of autoimmunity to GAD in the development of IDDM.
- Publisher
- AMER DIABETES ASSOC
- Keywords
- PURIFICATION; AUTOANTIGEN; CLONING; BRAIN; IDENTIFICATION; PROTEINS; SEQUENCE; EPITOPES; CDNA
- Publisher's Version
- https://doi.org/10.2337/diabetes.41.9.1182
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-09-01 12:00:00