REPRODUCIBILITY OF ESTIMATING 1ST PHASE INSULIN RESPONSES TO INTRAVENOUS GLUCOSE
- Author(s)
- KOSCHMANN, M; ALFORD, FP; Ward, GM; WALTERS, J; Colman, PG; Harrison, LC;
- Details
- Publication Year 1992-06,Volume 5,Issue #2,Page 73-79
- Journal Title
- DIABETES NUTRITION & METABOLISM
- Publication Type
- Journal Article
- Abstract
- Recent studies have questioned the reproducibility of first phase insulin response (FPIR) to intravenous glucose. The aim of the present study was to determine the variation of FPIR to intravenous glucose employing the following criteria: 1) the effect of single cannulation on estimating FPIR (between-arm study), 2) the reproducibility of FPIR when measured in the same individual on separate days (between-day study) and 3) the analysis of quantifying FPIR using four different mathematical methods i.e. 1+3 min insulin, mean DELTA-3-5 min insulin, DELTA-0-10 min insulin and DELTA-0-10 min insulin corrected for DELTA-0-10 min glucose. Ten normal individuals underwent two intravenous glucose tolerance tests (IVGTT) separated by 2-16 weeks. Dextrose 25% was administered over 60 seconds. For the between-arm study venous samples were collected simultaneously from the glucose infused arm and the contralateral arm. In the between-arm study, the within-subject mean coefficient of variation for the 1+3 min insulin was 11% (median 7.6%), mean DELTA-3-5 min insulin 19.7% (median 7.0%), DELTA-0-10 min insulin 7.9% (median 4.7%) and for the DELTA-0-10 min insulin/glucose area 8.7% (median 4.7%). Two subjects showed a large variation of glucose and insulin concentrations between the two arms over the initial 2-5 min. In the between-day study the within-subject mean coefficient of variation for the 1+3 min insulin was 30% (median 18%), mean DELTA-3-5 min insulin 29% (median 21.8%), DELTA-0-10 min insulin 26% (median 13%) and for the DELTA-0-10 min insulin/glucose area 20.3% (median 8.4%). It is concluded that 1) caution is required if venous sampling occurs from the glucose infused arm; 2) the reproducibility of glucose-normalised DELTA-0-10 min FPIR is acceptable for individual serial studies and 3) the diversity of the results for the four different mathematical methods for calculating FPIR supports the need for standardisation of the IVGTT procedure and estimation of FPIR.
- Publisher
- EDITRICE KURTIS S R L
- Keywords
- ISLET CELL ANTIBODIES; PRECISION; TOLERANCE
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1992-06-01 12:00:00