THE SURFACE PHENOTYPE OF DENDRITIC CELLS PURIFIED FROM MOUSE THYMUS AND SPLEEN - INVESTIGATION OF THE CD8 EXPRESSION BY A SUBPOPULATION OF DENDRITIC CELLS
- Author(s)
- Vremec, D; ZORBAS, M; Scollay, R; SAUNDERS, DJ; ARDAVIN, CF; Wu, L; Shortman, K;
- Details
- Publication Year 1992-07-01,Volume 176,Issue #1,Page 47-58
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- A new procedure for rapid isolation of dendritic cells (DC) was devised, involving collagenase digestion of tissues, dissociation of lymphoid-DC complexes, selection of light-density cells, then depletion of lymphocytes and other non-DC by treatment with a mixture of lineage-specific monoclonal antibodies (mAbs) and removal with antiimmunoglobulin-coupled magnetic beads. This enriched population (approximately 80% DC) was further purified when required by fluorescence-activated cell sorting for cells expressing high levels of class II major histocompatibility complex (MHC). The isolated DC were characterized by immunofluorescent staining using a panel of 30 mAbs. Thymic DC were surface positive for a number of markers characteristic of T cells, but they were distinct from T-lineage cells in expressing high levels of class II MHC, in lacking expression of the T cell receptor (TCR)-CD3 complex, and having TCR-beta and gamma genes in germline state. Splenic DC shared many markers with thymic DC, but were negative for most T cell markers, with the exception of CD8. A substantial proportion of DC from both thymus and spleen expressed CD8 at high levels, comparable with that on T cells. This appeared to be authentic CD8, and was produced by the DC themselves, since they contained CD8-alpha mRNA. Thymic DC presented both the CD8-alpha and beta chains on the cell surface (Ly-2+3+), although the alpha chain was in excess; the splenic DC expressed only the CD8-alpha chain (Ly-2+3-). It is suggested that the expression of CD8 could endow certain antigen-presenting DC with a veto function.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- MONOCLONAL-ANTIBODIES; DIFFERENTIATION ANTIGENS; LANGERHANS CELLS; LYMPHOID-CELLS; STROMAL CELLS; STEM-CELLS; T-CELLS; MACROPHAGE; RECEPTOR; LOCALIZATION
- Publisher's Version
- https://doi.org/10.1084/jem.176.1.47
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- Refer to copyright notice on published article.
Creation Date: 1992-07-01 12:00:00