PRODUCTION OF LEUKEMIA INHIBITORY FACTOR BY HUMAN ARTICULAR CHONDROCYTES AND CARTILAGE IN RESPONSE TO INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA
Publication Year 1993-06, Volume 36, Issue #6, Page 790-794
- Journal Title
- ARTHRITIS AND RHEUMATISM
- Publication Type
- Journal Article
- Objective. To investigate the production of leukemia inhibitory factor (LIF) by human articular chondrocytes and cartilage. Methods. Chondrocytes and cartilage were cultured with and without added cytokines, and the conditioned media assayed for LIF by a specific radioreceptor competition assay. Results. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) stimulated LIF production by chondrocyte monolayers and cartilage. LIF was generally not detected in unstimulated cultures. Northern blot analysis showed increased amounts of LIF messenger RNA in IL-1- and TNFalpha-treated chondrocyte cultures. Conclusion. Chondrocytes, stimulated by IL-1 and/or TNFalpha, are potential contributors to the elevated levels of LIF observed in the synovial fluids of patients with rheumatoid arthritis and other inflammatory arthritides.
- LIPPINCOTT-RAVEN PUBL
- RECOMBINANT HUMAN INTERLEUKIN-1; PLASMINOGEN-ACTIVATOR; PROSTAGLANDIN-E2; EXPRESSION; RESORPTION; IL-1
- Publisher's Version
- Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1993-06-01 12:00:00Last Modified: 0001-01-01 12:00:00