PRODUCTION OF LEUKEMIA INHIBITORY FACTOR BY HUMAN ARTICULAR CHONDROCYTES AND CARTILAGE IN RESPONSE TO INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA
Details
Publication Year 1993-06, Volume 36, Issue #6, Page 790-794
Journal Title
ARTHRITIS AND RHEUMATISM
Publication Type
Journal Article
Abstract
Objective. To investigate the production of leukemia inhibitory factor (LIF) by human articular chondrocytes and cartilage. Methods. Chondrocytes and cartilage were cultured with and without added cytokines, and the conditioned media assayed for LIF by a specific radioreceptor competition assay. Results. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) stimulated LIF production by chondrocyte monolayers and cartilage. LIF was generally not detected in unstimulated cultures. Northern blot analysis showed increased amounts of LIF messenger RNA in IL-1- and TNFalpha-treated chondrocyte cultures. Conclusion. Chondrocytes, stimulated by IL-1 and/or TNFalpha, are potential contributors to the elevated levels of LIF observed in the synovial fluids of patients with rheumatoid arthritis and other inflammatory arthritides.
Publisher
LIPPINCOTT-RAVEN PUBL
Keywords
RECOMBINANT HUMAN INTERLEUKIN-1; PLASMINOGEN-ACTIVATOR; PROSTAGLANDIN-E2; EXPRESSION; RESORPTION; IL-1
Rights Notice
Refer to copyright notice on published article.


Creation Date: 1993-06-01 12:00:00
Last Modified: 0001-01-01 12:00:00
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