PRODUCTION OF LEUKEMIA INHIBITORY FACTOR BY HUMAN ARTICULAR CHONDROCYTES AND CARTILAGE IN RESPONSE TO INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA

Author(s): Campbell, IK; Waring, P; Novak, U; Hamilton, JA;
Details: Publication Year 1993-06,Volume 36,Issue #6,Page 790-794
Journal Title: ARTHRITIS AND RHEUMATISM
Publication Type: Journal Article
Abstract: Objective. To investigate the production of leukemia inhibitory factor (LIF) by human articular chondrocytes and cartilage. Methods. Chondrocytes and cartilage were cultured with and without added cytokines, and the conditioned media assayed for LIF by a specific radioreceptor competition assay. Results. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) stimulated LIF production by chondrocyte monolayers and cartilage. LIF was generally not detected in unstimulated cultures. Northern blot analysis showed increased amounts of LIF messenger RNA in IL-1- and TNFalpha-treated chondrocyte cultures. Conclusion. Chondrocytes, stimulated by IL-1 and/or TNFalpha, are potential contributors to the elevated levels of LIF observed in the synovial fluids of patients with rheumatoid arthritis and other inflammatory arthritides.
Publisher: LIPPINCOTT-RAVEN PUBL
Keywords: RECOMBINANT HUMAN INTERLEUKIN-1; PLASMINOGEN-ACTIVATOR; PROSTAGLANDIN-E2; EXPRESSION; RESORPTION; IL-1
Publisher's Version: https://doi.org/10.1002/art.1780360608
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1993-06-01 12:00:00