DEVELOPMENT OF GAMMA-DELTA T-CELLS IN THE ADULT MURINE THYMUS
Author(s)
ZORBAS, M; Scollay, R;
Details
Publication Year 1993-07,Volume 23,Issue #7,Page 1655-1660
Journal Title
EUROPEAN JOURNAL OF IMMUNOLOGY
Publication Type
Journal Article
Abstract
The development of T cells belonging to the gammadelta lineage is not well understood.We have analyzed the cells in the adult murine thymus which express the gammadelta TcR on the surface in order to learn more about this process. Our data demonstrate a number of clear subpopulations of gammadelta expressing cells in the thymus based on the expression of Thy-1 and HSA (heat-stable antigen). Only one of these subpopulations, the one expressing both Thy-1 and HSA, contains dividing cells or has a significant rate of turnover. Together with the fact that emigrant gammadelta cells are HSA+Thy-1+, this suggests that this thymic subpopulation is the sole, or major, source of exported cells. However, the turnover of cells from this population is 5 x 10(4) - 10 x 10(4) cells per day, while previous estimates of the rate of export of gammadelta cells are in the order of 10(4) cells per day. Furthermore the Vgamma profile of recent gammadelta+ emigrants differs from that of the thymic HSA+Thy-1+ cells. This raises the possibility that only a selected subpopulation of the thymic gammadelta+HSA+Thy-1+ population is exported, and that some gammadelta cells may die in situ in the thymus. The function of the other gammadelta thymic subpopulations, which are turning over very slowly or not at all, (i.e. the HSA-Thy-1- and HSA-Thy-1+ subpopulations) remains unclear.
Publisher
VCH PUBLISHERS INC
Keywords
INTESTINAL INTRAEPITHELIAL LYMPHOCYTES; DENDRITIC EPIDERMAL-CELLS; ALPHA-BETA; MONOCLONAL-ANTIBODIES; DIFFERENTIATION ANTIGENS; LIMITED DIVERSITY; RECEPTOR; THYMOCYTES; EXPRESSION; BEARING
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Creation Date: 1993-07-01 12:00:00
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