SCL, THE GENE IMPLICATED IN HUMAN T-CELL LEUKEMIA, IS ONCOGENIC IN A MURINE T-LYMPHOCYTE CELL-LINE
- Author(s)
- Elwood, NJ; Cook, WD; Metcalf, D; Begley, CG;
- Details
- Publication Year 1993-11,Volume 8,Issue #11,Page 3093-3101
- Journal Title
- ONCOGENE
- Publication Type
- Journal Article
- Abstract
- SCL (TAL-1) is implicated in the generation of human T-cell acute lymphoblastic leukaemia. To directly examine the role of this putative oncogene, an SCL retrovirus was constructed and used to infect a v-ABL transformed T-lymphocyte cell line. Thirteen independent SCL-infected and four control cell lines were established and injected subcutaneously into syngeneic mice. Mice injected with SCL-infected clonal cell lines died significantly more rapidly than control animals. By day 200 46% (40/87) of animals injected with SCL-infected cell lines had died due to disseminated transplantable lymphoid tumours. In contrast only 22% of control mice were dead by day 200 (P < 0.0015). Of possible relevance to the enhanced tumourigenesis, some SCL-infected cell lines displayed increased clonogenicity in agar. Increased cell growth was even more striking when ex-vivo tumour-derived cell lines were studied. Thus, SCL can co-operate with v-ABL to hasten T-cell tumourigenesis. This is the first direct evidence demonstrating that SCL can behave as an oncogene.
- Publisher
- STOCKTON PRESS
- Keywords
- ABELSON LEUKEMIA-VIRUS; DNA-BINDING MOTIF; LOOP-HELIX GENES; C-MYC; ERYTHROID-DIFFERENTIATION; CHROMOSOMAL TRANSLOCATION; TRANSGENIC MICE; BALB/C MICE; TAL-1 GENE; EXPRESSION
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Creation Date: 1993-11-01 12:00:00