TCR-BETA AND TCR-ALPHA GENE ENHANCERS CONFER TISSUE-SPECIFICITY AND STAGE-SPECIFICITY ON V(D)J RECOMBINATION EVENTS
- Author(s)
- CAPONE, M; WATRIN, F; FERNEX, C; HORVAT, B; KRIPPL, B; Wu, L; Scollay, R; FERRIER, P;
- Details
- Publication Year 1993-11,Volume 12,Issue #11,Page 4335-4346
- Journal Title
- EMBO JOURNAL
- Publication Type
- Journal Article
- Abstract
- We describe transgenic mice carrying germline variable gene segments associated with either the T cell receptor (TCR) beta or alpha gene enhancers (Ebeta or Ealpha). Transgenic constructs underwent high rates of site-specific rearrangements predominantly in T cells from independent mice. Rearrangements of the Ebeta-containing transgenes began at different stages of T cell differentiation in embryonic and adult thymus than did the Ealpha-containing ones, with a pattern superimposable upon the patterns of TCRbeta or TCRalpha gene expression, respectively. We demonstrate that sequences within the TCRbeta and TCRalpha gene enhancers confer tissue- and stage-specificity upon the V(D)J recombination events affecting adjacent gene segments. The patterns of transgene expression also gave information on developmental events and lineage relationships (gammadelta versus alphabeta) during T cell development.
- Publisher
- OXFORD UNIV PRESS UNITED KINGDOM
- Keywords
- T-CELL RECEPTOR; NUCLEAR PROTEINS INTERACT; ADULT MURINE THYMUS; TRANSCRIPTIONAL ENHANCER; REGULATORY ELEMENTS; TRANSGENIC MICE; CHAIN GENES; HMG DOMAIN; C-ALPHA; 3' END
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Creation Date: 1993-11-01 12:00:00