MOUSE THYMIC DENDRITIC CELL SUBPOPULATIONS
- Author(s)
- ARDAVIN, C; Wu, L; Ferrero, I; Shortman, K;
- Details
- Publication Year 1993-09,Volume 38,Issue #1,Page 19-25
- Journal Title
- IMMUNOLOGY LETTERS
- Publication Type
- Journal Article
- Abstract
- Mouse thymic dendritic cells (DC) have been isolated after collagenase digestion, selection of the low-density cell fraction, then depletion of T-lineage cells and other non-DC by treatment with specific monoclonal antibodies (mAb) and removal with anti-Ig-coated magnetic beads. The resulting DC preparation represented 0.1-0.2% of total thymic cells and contained 70-80% DC. Flow cytometry analysis of MHC class II (MHC II) expression by DC showed that 40% of DC expressed intermediate levels of MHC II, and 60% expressed high levels of this marker. Moreover, immunofluorescent 2-colour staining allowed the characterization of two clearly distinguishable DC subpopulations: MHC II(inter) DC were CD45hi, CD44hi, HSA(hi), whereas MHC II(hi) DC were CD45lo, CD44lo, HSA(lo). These results are discussed with regard to the functional significance of MHC II(inter) and MHC II(hi) DC subpopulations in the mouse thymus.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- SURFACE
- Publisher's Version
- https://doi.org/10.1016/0165-2478(93)90113-G
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1993-09-01 12:00:00