TRANSCRIPTION FACTOR JUN-B IS TARGET OF AUTOREACTIVE T-CELLS IN IDDM
- Author(s)
- Honeyman, MC; Cram, DS; Harrison, LC;
- Details
- Publication Year 1993-04,Volume 42,Issue #4,Page 626-630
- Journal Title
- DIABETES
- Publication Type
- Journal Article
- Abstract
- Target antigens defined by autoantibodies in IDDM include insulin, a putative glycolipid that reacts with islet cell antibodies, and a 64,000-M(r) protein recently identified as glutamic acid decarboxylase. In addition, some IDDM sera that contain antibodies to glutamic acid decarboxylase also coprecipitate a 38,000-M(r) protein from islets. This study used a high titer anti-38,000-M(r) serum to screen bacteriophage lambda cDNA expression libraries and identified human islet and placental clones encoding jun-B, the nuclear transcription protein, of predicted 38,000 M(r). Peripheral blood T-cells exhibited significant proliferation in response to a recombinant fragment of jun-B (amino acids 1-180) in 12 of 17 (71%) recent-onset IDDM subjects, 8 of 16 (50%) ICA-positive first-degree relatives of IDDM subjects who were at risk, 3 of 12 (25%) other autoimmune disease subjects, and 0 of 10 healthy control subjects. Proliferation to tetanus toxoid did not differ significantly between the groups. Responses to jun-B were not related to age, sex, or human leukocyte antigen status. Thus, autoreactive T-cells identify a novel antigen, p38 jun-B, in IDDM and appear to indicate subjects at risk for the development of clinical disease.
- Publisher
- AMER DIABETES ASSOC
- Keywords
- INSULIN-SECRETORY-GRANULE; GENE-EXPRESSION; EARLY RESPONSE; BETA-CELL; ANTIBODIES; PROTEINS; FOS; AUTOANTIGEN; REACTIVITY; CLONES
- Publisher's Version
- https://doi.org/10.2337/diabetes.42.4.626
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1993-04-01 12:00:00