EMIGRATION OF SELECTED SUBSETS OF GAMMA-DELTA+ T-CELLS FROM THE ADULT MURINE THYMUS
- Author(s)
- Kelly, KA; Pearse, M; Lefrancois, L; Scollay, R;
- Details
- Publication Year 1993-04,Volume 5,Issue #4,Page 331-335
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Cells bearing the gammadelta form of the TCR make up only 1 - 3% of T cells in the adult murine thymus and peripheral lymphoid organs. Evidence from studies of nude mice suggests that the development of at least some gammadelta T cells is thymus dependent; however, until now it has not been directly demonstrated that gammadelta cells are exported from the thymus. In this paper we have used the technique of labelling thymocytes in vivo with FITC, followed by flow cytometric analysis to trace cells emigrating from the thymus to the spleen. Using this approach we have been able to demonstrate for the first time that gammadelta T cells are exported from the adult murine thymus to the spleen. We also demonstrate that the cells emigrating to the spleen are a selected subset of gammadelta thymocytes being heat stable antigen positive, Thy-1+, and expressing low levels of CD44 (Pgp-1). In addition, investigation of TCR V(gamma) gene usage among adult gammadelta+ thymocytes, recent emigrants, and spleen cells, indicated a selective emigration of gammadelta cells expressing certain V(gamma) genes.
- Publisher
- OXFORD UNIV PRESS UNITED KINGDOM
- Keywords
- INTESTINAL INTRAEPITHELIAL LYMPHOCYTES; MONOCLONAL-ANTIBODIES; ATHYMIC MICE; DIFFERENTIATION ANTIGENS; RECEPTOR; EXPRESSION; THYMOCYTES; BEARING; GENES; CD4
- Publisher's Version
- https://doi.org/10.1093/intimm/5.4.331
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1993-04-01 12:00:00