MONOCLONAL-ANTIBODIES THAT RECOGNIZE HUMAN GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR AND NEUTRALIZE ITS BIOACTIVITY INVITRO
- Author(s)
- DEMPSEY, PJ; Layton, JE; DUHRSEN, U; Nicola, NA; Cebon, J; Burgess, AW; MORSTYN, G;
- Details
- Publication Year 1990-12,Volume 9,Issue #6,Page 545-558
- Journal Title
- HYBRIDOMA
- Publication Type
- Journal Article
- Abstract
- We have produced monoclonal antibodies to bacterially synthesized, human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and have studied in detail the characteristics of three strongly neutralizing antibodies. The antibodies reacted with GM-CSF at high dilution (EC50 = 0.1-1.7 nM) in an indirect ELISA but did not react with murine GM-CSF or other cytokines. They also recognized glycosylated hGM-CSF produced by human lymphocytes. The antibodies were able to immunoprecipitate rhGM-CSF, but only reacted weakly with rhGM-CSF on Western blots, indicating that they recognized a conformation-dependent epitope. Cross-blocking studies showed that the three antibodies recognized overlapping epitopes. The antibodies inhibited binding of I-125-labeled rhGM-CSF to HL-60 cells at nanomolar concentrations and neutralized GM-CSF activity in two different bioassays. These antibodies thus provide a useful tool for analyzing the specificity of bioassays and for further studies of the production and function of GM-CSF in vitro and in vivo.
- Publisher
- MARY ANN LIEBERT INC PUBL
- Keywords
- HUMAN GM-CSF; GROWTH; IMMUNOASSAY; PROTEINS; CLONING; BINDING; CELLS; DNA
- Publisher's Version
- https://doi.org/10.1089/hyb.1990.9.545
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1990-12-01 12:00:00