DISTRIBUTION, ISOLATION AND SEQUENCE-ANALYSIS OF THE C-TERMINAL HEPTAPEPTIDE OF PRO-ENKEPHALIN-A (YGGFMRF) FROM THE OVINE MEDIAN-EMINENCE

Author(s): Giraud, AS; Clarke, IJ; RUNDLE, SE; PARKER, LM; FUNDER, JW; Simpson, RJ; SMITH, AI;
Details: Publication Year 1991,Volume 3,Issue #2,Page 215-220
Journal Title: JOURNAL OF NEUROENDOCRINOLOGY
Publication Type: Journal Article
Abstract: Using a polyclonal antiserum raised against the C-terminal heptapeptide of pro-enkephalin A, we have isolated the opioid heptapeptide Tyr-Gly-Gly-Phe-Met-Arg-Phe (MERF) from ovine median eminence and mapped its distribution in that structure. MERF-immunoreactivity was confined to the pars externa (neurosecretory zone) where it colocalized with corticotrophin-releasing factor in the majority of terminals. No larger, N-terminally extended forms of MERF were detected in median eminence extracts suggesting that pro-enkephalin is fully processed to its constituent enkephalin congeners, and that the bioactive products, including MERF, act at the level of the hypothalamus in regulating anterior pituitary function.
Publisher: BLACKWELL SCIENCE LTD
Keywords: MET-ENKEPHALIN; OPIATE RECEPTORS; RAT NEUROHYPOPHYSIS; BIOLOGICAL-ACTIVITY; NERVE-TERMINALS; MOLECULAR-FORMS; OPIOID-PEPTIDES; GROWTH-HORMONE; RELEASE; PROENKEPHALIN
Publisher's Version: https://doi.org/10.1111/j.1365-2826.1991.tb00265.x
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1991-01-01 12:00:00