ACTIVATION OF HUMAN LYMPHOCYTES-T BY LEISHMANIA LIPOPHOSPHOGLYCAN
- Kemp, M; Theander, TG; Handman, E; HEY, AS; KURTZHALS, JAL; HVIID, L; SORENSEN, AL; WERE, JOB; KOECH, DK; Kharazmi, A;
Publication Year 1991-02, Volume 33, Issue #2, Page 219-224
- Journal Title
- SCANDINAVIAN JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.
- BLACKWELL SCIENCE LTD
- EXPERIMENTAL VISCERAL LEISHMANIASIS; CUTANEOUS LEISHMANIASIS; INTERFERON-GAMMA; IMMUNIZATION; MACROPHAGES; RECEPTOR; INTERLEUKIN-2; MONOCYTES; DONOVANI; CELLS
- Publisher's Version
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- Refer to copyright notice on published article.
Creation Date: 1991-02-01 12:00:00Last Modified: 0001-01-01 12:00:00