ISOLATION OF CDNA-ENCODING A NEWLY IDENTIFIED MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN - INTRACELLULAR TARGETING TO THE AMYLOPLAST
- Author(s)
- Singh, MB; HOUGH, T; THEERAKULPISUT, P; AVJIOGLU, A; DAVIES, S; SMITH, PM; TAYLOR, P; Simpson, RJ; Ward, LD; McCluskey, J; PUY, R; KNOX, RB;
- Details
- Publication Year 1991-02,Volume 88,Issue #4,Page 1384-1388
- Journal Title
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Publication Type
- Journal Article
- Abstract
- We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31 kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.
- Publisher
- NATL ACAD SCIENCES
- Keywords
- LOLIUM-PERENNE POLLEN; MONOCLONAL-ANTIBODIES; PURIFICATION
- Publisher's Version
- https://doi.org/10.1073/pnas.88.4.1384
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-02-01 12:00:00