DISTRIBUTION AND COMPARISON OF RECEPTORS FOR LEUKEMIA INHIBITORY FACTOR ON MURINE HEMATOPOIETIC AND HEPATIC CELLS
- Author(s)
- Hilton, DJ; Nicola, NA; Metcalf, D;
- Details
- Publication Year 1991-02,Volume 146,Issue #2,Page 207-215
- Journal Title
- JOURNAL OF CELLULAR PHYSIOLOGY
- Publication Type
- Journal Article
- Abstract
- Leukemia inhibitory factor (LIF) is a glycoprotein that induces the differentiation of the monocytic leukemia cell line M1 but suppresses the differentiation of totipotent embryonic stem cells. In an attempt to define the normal cellular targets for LIF, the distribution of LIF receptors within hemopoietic and hepatic tissue was analyzed by binding cells with radioiodinated LIF (I-125-LIF) and subsequently carrying out autoradiography. Autoradiography demonstrated that in each hemopoietic tissue examined cells of monocyte/macrophage lineage were the primary cell type labeled with I-125-LIF. Moreover, both fetal and adult parenchymal hepatocytes displayed higher levels of labeling than either monocytes or macrophages. The number of receptors per positive cell varied from 150 for bone marrow monocytes to 2,000 for adult hepatocytes. In each case, however, binding was of high affinity, with an apparent K(D) of 34-100 pM, and binding was specific, since labeling was competed for by unlabeled LIF but not a range of other structurally unrelated growth and differentiation factors. It is suggested that LIF may play a role in regulating macrophage function and hepatic acute phase protein synthesis in response to infection.
- Publisher
- WILEY-LISS
- Keywords
- COLONY-STIMULATING FACTOR; FACTOR INDUCING DIFFERENTIATION; EMBRYONIC STEM-CELLS; KREBS ASCITES-CELLS; RAT-LIVER CELLS; MYELOID-LEUKEMIA; CONDITIONED MEDIUM; HEMATOPOIETIC-CELLS; MOLECULAR-CLONING; PURIFICATION
- Publisher's Version
- https://doi.org/10.1002/jcp.1041460204
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-02-01 12:00:00