CLONING AND SEQUENCING OF LOL PI, THE MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN
- Author(s)
- GRIFFITH, IJ; SMITH, PM; POLLOCK, J; THEERAKULPISUT, P; AVJIOGLU, A; DAVIES, S; HOUGH, T; Singh, MB; Simpson, RJ; Ward, LD; KNOX, RB;
- Details
- Publication Year 1991-02-25,Volume 279,Issue #2,Page 210-215
- Journal Title
- FEBS LETTERS
- Publication Type
- Journal Article
- Abstract
- We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- PERENNE POLLEN; MONOCLONAL-ANTIBODIES; COMPONENTS; HLA-DR3; ASTHMA; PLANTS
- Publisher's Version
- https://doi.org/10.1016/0014-5793(91)80151-R
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-02-25 12:00:00