FIBROBLAST GROWTH FACTOR-MEDIATED PROLIFERATION OF CENTRAL-NERVOUS-SYSTEM PRECURSORS DEPENDS ON ENDOGENOUS PRODUCTION OF INSULIN-LIKE GROWTH FACTOR-I

Author(s): DRAGO, J; Murphy, M; CARROLL, SM; Harvey, RP; Bartlett, PF;
Details: Publication Year 1991-03,Volume 88,Issue #6,Page 2199-2203
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.
Publisher: NATL ACAD SCIENCES
Keywords: RAT-BRAIN; CELL-PROLIFERATION; DNA-SYNTHESIS; FETAL BRAIN; MOUSE; SOMATOMEDINS; EXPRESSION; RECEPTORS; ONCOGENE; CULTURES
Publisher's Version: https://doi.org/10.1073/pnas.88.6.2199
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1991-03-01 12:00:00