FIBROBLAST GROWTH FACTOR-MEDIATED PROLIFERATION OF CENTRAL-NERVOUS-SYSTEM PRECURSORS DEPENDS ON ENDOGENOUS PRODUCTION OF INSULIN-LIKE GROWTH FACTOR-I
Details
Publication Year 1991-03,Volume 88,Issue #6,Page 2199-2203
Journal Title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type
Journal Article
Abstract
Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.
Publisher
NATL ACAD SCIENCES
Keywords
RAT-BRAIN; CELL-PROLIFERATION; DNA-SYNTHESIS; FETAL BRAIN; MOUSE; SOMATOMEDINS; EXPRESSION; RECEPTORS; ONCOGENE; CULTURES
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 1991-03-01 12:00:00
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