DIRECT EVIDENCE THAT GANGLIOSIDE IS AN INTEGRAL COMPONENT OF THE THYROTROPIN RECEPTOR

KIELCZYNSKI, W; Harrison, LC; LEEDMAN, PJ

Author(s): KIELCZYNSKI, W; Harrison, LC; LEEDMAN, PJ;
Details: Publication Year 1991-03,Volume 88,Issue #5,Page 1991-1995
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Gangliosides were extracted from purified human and porcine thyrotropin (TSH) receptors (TSH-R) and were detected by probing with an I-125-labeled sialic acid-specific lectin, Limax flavus agglutinin. Gangliosides copurified with human and porcine TSH-R migrated between monosialoganglioside GM1 and disialoganglioside GD1a. Ceramide glycanase digestion of the purified human TSH-R-associated glycolipid confirmed its ganglioside nature. It was resistant to Vibrio cholerae sialidase, which digests all gangliosides except GM1, but was sensitive to Arthrobacter ureafaciens sialidase, which digests all gangliosides including GM1. These findings indicate that the human TSH-R contains ganglioside that belongs to the galactosyl(beta-1 --> 3)-N-acetylgalactosaminyl(beta-1 --> 4)-[N-acetylneuraminyl(alpha-2 --> 3)]galactosyl(beta-1 --> 4)glucosyl(beta-1 --> 1)ceramide (GM1) family. Its intimate association with receptor protein implies a key role for ganglioside in the structure and function of the TSH-R.
Publisher: NATL ACAD SCIENCES
Keywords: PLASMA-MEMBRANES; BINDING PROTEINS; THYROID-CELLS; SURFACE; GLYCOSPHINGOLIPIDS; IMMUNOGLOBULINS; GLYCOPROTEIN; AFFINITY; ANTIBODY; TUMOR
Publisher's Version: https://doi.org/10.1073/pnas.88.5.1991
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1991-03-01 12:00:00