STRUCTURE AND EXPRESSION OF MOUSE GERMLINE IMMUNOGLOBULIN-GAMMA-3 HEAVY-CHAIN TRANSCRIPTS INDUCED BY THE MITOGEN LIPOPOLYSACCHARIDE
- Author(s)
- Gerondakis, S; GAFF, C; Goodman, DJ; Grumont, RJ;
- Details
- Publication Year 1991-12,Volume 34,Issue #6,Page 392-400
- Journal Title
- IMMUNOGENETICS
- Publication Type
- Journal Article
- Abstract
- Germline immunoglobulin heavy chain gene transcription is though to direct isotype switching by modulating the accessibility of specific switch regions to a recombinase. In this study, cloned cDNA copies of mouse germline Igh-8 RNAs have been used to characterize the Igh-8 transcription unit. The 5' end of these transcripts are derived from an exon denoted Ig3, located 1 kilobase 5' of the Igh-8 switch region. Sequence analysis of cDNA and genomic clones reveals that these RNAs are noncoding. In splenic B cell cultures treated with lipopolysaccharide (LPS), germline Igh-8 transcript levels are upregulated after 8 h due to increased transcription. This induction is consistent with the identification of a putative binding site for the LPS inducible transcription factor NF-kappa-B approximately 150 nucleotides upstream of the sites of transcript initiation. Furthermore, nucleotide sequence comparisons reveal that the region encompassing the site of germline Igh-8 transcription initiation is highly homologous to part of the Ig2b exon, and is also conserved upstream of the Igh-1 switch region. The implications of these findings for the control of germline Igh-8 transcription is discussed.
- Publisher
- SPRINGER VERLAG
- Keywords
- MURINE-B-CELLS; CONSTANT-REGION GENE; NUCLEOTIDE-SEQUENCE; SWITCH REGIONS; MESSENGER-RNA; C-EPSILON; INDUCTION; INTERLEUKIN-4; GAMMA; RECOMBINATION
- Publisher's Version
- https://doi.org/10.1007/BF01787490
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-12-01 12:00:00