THE SURVIVAL OF XENOGRAFTS, ALLOGRAFTS AND ISOGRAFTS IN NOD MICE, AND XENOGRAFTS IN OTHER STRAINS, AFTER IMMUNOSUPPRESSION WITH ANTI-CD4 MONOCLONAL-ANTIBODY
- Author(s)
- Mandel, TE; Koulmanda, M;
- Details
- Publication Year 1992,Volume 5,Issue #3,Page 91-96
- Journal Title
- DIABETES NUTRITION & METABOLISM
- Publication Type
- Journal Article
- Abstract
- Transplants of organ-cultured fetal pancreas were performed in NOD mice that are prone to develop diabetes, and in other strains not susceptible to autoimmune disease. In some NOD mice, simultaneous grafts of isogeneic fetal NOD pancreas and allogeneic CBA pancreas were compared with xenografts of fetal pig pancreas. In prediabetic NOD/Wehi male mice that were immunosuppressed with an anti-CD4 MAb (GK1.5) in the peritransplant period (days -1, 0 and +1), there was preferential survival of the xenografts, while isografts showed infiltration suggestive of recurrent disease, and allografts were rapidly rejected. In contrast, in NOD/Lt female mice that develop a high incidence of spontaneous diabetes (>90% at 250 d), xenograft survival was shortened, but fetal pig islets could be retained for long periods when weekly GK1.5 was given. CBA and BALB/c mice also showed good survival of pig islets to 28 days after peritransplant treatment. Organ culture in high concentrations of 02 enhanced xenograft survival in NOD/Lt female mice, if the animals were given an extended course of anti-CD4 treatment, but pig islets not immunomodified were rapidly rejected in such recipients. Thus, recipient immunosuppression, strain, sex and mode of preculture graft preparation all play a role in xenograft survival.
- Publisher
- EDITRICE KURTIS S R L
- Keywords
- PIG PROISLET XENOGRAFTS; PANCREAS TRANSPLANTATION; MARKED PROLONGATION; ANTILYMPHOCYTE SERA; DIABETES-INCIDENCE; MOUSE; REJECTION; MELLITUS; CULTURE
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Creation Date: 1992-01-01 12:00:00