LYMPHOKINE REQUIREMENTS FOR THE DEVELOPMENT OF SPECIFIC CYTOTOXIC T-CELLS FROM SINGLE PRECURSORS
- Author(s)
- ROGERS, LA; ZLOTNIK, A; LEE, F; Shortman, K;
- Details
- Publication Year 1991-04,Volume 21,Issue #4,Page 1069-1072
- Journal Title
- EUROPEAN JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- A high cloning efficiency, filler cell-free culture system was developed for the growth of single murine cytotoxic T lymphocyte precursors (CTLp) and their differentiation into cytotoxic T lymphocytes (CTL). The system used nonspecific stimulation with phorbol ester and calcium ionophore in the presence of recombinant lymphokines. The optimal lymphokine combination was interleukin 2 throughout, together with interferon-gamma during the first 6 days and interleukin 6 during the last 2 days of culture. Under these conditions half of all CD4-CD8+ T cells became CTL clones. The CTL were CD4-CD8+CD3+ TcR-alpha/beta+ and were derived from CD4-CD8+Pgp-1- precursors.
- Publisher
- VCH PUBLISHERS INC
- Keywords
- CYTO-TOXIC LYMPHOCYTES; STIMULATORY FACTOR-I; DIFFERENTIATION; IDENTIFICATION; INTERLEUKIN-2; INTERFERON; THYMOCYTES; INDUCTION; CULTURES; SIGNAL
- Publisher's Version
- https://doi.org/10.1002/eji.1830210432
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-04-01 12:00:00