HIGH-FREQUENCY ACTIVATION OF SINGLE CD4+ AND CD8+ T-CELLS TO PROLIFERATE AND SECRETE CYTOKINES USING ANTIRECEPTOR ANTIBODIES AND IL-2(1)
- Author(s)
- Maraskovsky, E; PECH, MH; Kelso, A;
- Details
- Publication Year 1991-03,Volume 3,Issue #3,Page 255-264
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- A high cloning efficiency single-cell culture system was developed to define the activation requirements of isolated CD4+ and CD8+ T cells to proliferate and secrete cytokines. T cells were triggered using solid-phase anti-CD3 and anti-CD4 or anti-CD8 antibodies plus rIL-2. Activation was measured by microscopic scoring of proliferation and by measurement of cytokine production using the cytokine-responsive cell lines FDC-P1, which responds to GM-CSF, IL-3, IFN-gamma and IL-4, and 32D clone 3 which responds to IL-3 only. Whilst anti-CD3 plus rIL-2 triggered only 4% of peripheral T cells to proliferate, anti-CD3 plus anti-CD8 mAb triggered about 40% of CD8+ T cells; 80% of the resultant clones secreted cytokine and 90% of these were IL-3+. Anti-CD3 plus anti-CD4 mAb triggered proliferation in about 20% of CD4+ T cells, of which 34% formed cytokine-producing clones with 47% of these secreting IL-3. In addition to responding at higher frequency, CD8+ T cells formed larger clones which produced higher levels of cytokines than CD4+ cells. Cell separation on the basis of Pgp-1 expression suggested that this culture system did not select for previously activated cells. Whereas Pgp-1+ T cells from keyhole limpet haemocyanin (KLH)-primed mice were enriched in KLH-specific cells, no significant differences were observed in the clonogenicity or cytokine-secreting capacity of Pgp-1+ and Pgp-1- T cells from normal mice.
- Publisher
- OXFORD UNIV PRESS UNITED KINGDOM
- Keywords
- COLONY-STIMULATING FACTOR; CYTO-TOXIC LYMPHOCYTES; TYROSINE-PROTEIN-KINASE; CROSS-LINKING; MHC MOLECULES; INTERFERON; CLONES; INTERLEUKIN-2; COMPLEX; EXPRESSION
- Publisher's Version
- https://doi.org/10.1093/intimm/3.3.255
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-03-01 12:00:00