DETECTION OF CYTOMEGALOVIRUS BY THE POLYMERASE CHAIN-REACTION - A SIMPLE, RAPID AND SENSITIVE NONRADIOACTIVE METHOD
- Author(s)
- GREENFIELD, C; SINICKAS, V; Harrison, LC;
- Details
- Publication Year 1991-03-18,Volume 154,Issue #6,Page 383-385
- Journal Title
- MEDICAL JOURNAL OF AUSTRALIA
- Publication Type
- Journal Article
- Abstract
- Cytomegalovirus (CMV) is an important pathogen in persons who are immunocompromised or who have received organ transplants. The rate of symptomatic CMV infection is relatively higher after liver transplantation, and CMV hepatitis may be difficult to differentiate from rejection or cholangitis without recourse to liver biopsy and tissue culture. In this study we have used the polymerase chain reaction (PCR) to amplify and detect CMV-DNA sequences directly from the saliva and urine of liver transplant recipients. The procedure is simple, rapid and specific, and was found to be as sensitive as tissue culture for the diagnosis of CMV.
- Publisher
- AUSTRALASIAN MED PUBL CO LTD
- Keywords
- HUMAN IMMUNODEFICIENCY VIRUS; PERIPHERAL-BLOOD; INFECTION; DNA; AMPLIFICATION; DIAGNOSIS
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-03-18 12:00:00