HOUSE DUST MITE-DERIVED AMYLASE - ALLERGENICITY AND PHYSICOCHEMICAL CHARACTERIZATION

LAKE, FR; Ward, LD; Simpson, RJ; Thompson, PJ; Stewart, GA

Author(s): LAKE, FR; Ward, LD; Simpson, RJ; Thompson, PJ; Stewart, GA;
Details: Publication Year 1991-06,Volume 87,Issue #6,Page 1035-1042
Journal Title: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
Publication Type: Journal Article
Abstract: Amylase activity was found in extracts of both Dermatophagoides pteronyssinus whole mite (0.16 U/mg) and spent growth medium (0.01 U/mg) but not in unused growth medium. It was also detected in all extracts of house dust obtained from mattresses (n = 20; geometric mean, 1.95 U/gm) and in 18 extracts of dust obtained from lounge room carpets (n = 20; geometric mean, 0.54 U/gm). Although the origins of amylase in dust are unclear, enzyme activity correlated with mite counts (n = 40; r = 0.35; p < 0.05) and Der p I concentrations (r = 0.41; p < 0.01). Mite amylase was purified from spent growth medium by affinity chromatography, gel filtration, and chromatofocusing. It was physicochemically similar to mammalian amylase with regard to molecular weight (60,000), charge heterogeneity (isoelectric point, 5 to 7) and the capacity to bind to an organomercurial affinity matrix. The optimum pH for enzymatic activity was revealed to be 6.4. IgE immunoblot studies demonstrated that the enzyme was allergenic and that its expression was dependent on the integrity of intrachain disulfide bonds. Sera from 25% of mite-allergic children and 46% of mite-allergic adults contained specific IgE to mite amylase. IgE to amylase was associated (p < 0.01) with increased concentrations of total mite-specific IgE determined with a direct RAST assay.
Publisher: MOSBY-YEAR BOOK INC
Keywords: DERMATOPHAGOIDES-PTERONYSSINUS; CROSSED RADIOIMMUNOELECTROPHORESIS; P-II; COMPONENTS; IDENTIFICATION; ANTIGENS; EXTRACTS; SEQUENCE; PROTEIN
Publisher's Version: https://doi.org/10.1016/0091-6749(91)92147-S
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1991-06-01 12:00:00