DOES NEGATIVE SELECTION INVOLVE ACCUMULATION OF SELF-REACTIVE THYMOCYTES IN THYMIC ROSETTES
- Author(s)
- Shortman, K; Vremec, D; LEES, RK; MACDONALD, HR;
- Details
- Publication Year 1991-06,Volume 28,Issue #3,Page 201-206
- Journal Title
- IMMUNOLOGY LETTERS
- Publication Type
- Journal Article
- Abstract
- Thymic rosettes, the natural associations between thymocytes and either macrophages or dendritic cells, were isolated from the thymus by collagenase digestion and unit-gravity elutriation. Rosettes from mouse strains where either the V-beta-6-bearing thymocytes are deleted because of reactivity with products of the Mls(a) allele of the minor lymphocyte stimulating locus, or where V-beta-17a-bearing thymocytes are deleted because of reactivity with IE class II MHC molecules, were compared with rosettes from appropriate control strains to test if a selective association with stromal cells preceded deletion. Rosettes from an Mls(a)-bearing strain were able to stimulate an Mls(a)-reactive T-hybridoma, but much of this stimulatory activity was attributable to the few B cells associated with the rosette preparations; the stromal components of the rosettes appeared to be poor presenters of Mls(a) gene products. There was no enrichment of thymocytes bearing high or low levels of V-beta-6 TcR in the rosettes from the Mls(a)-bearing strain, which might have reflected the poor presentation by the stromal cells. However, nor was there detectable selective association of thymocytes bearing C-beta-17a in the rosettes from an IE-positive mouse strain. This argues against binding and immobilisation on stromal cells as part of the deletion process, but not against the stromal cells delivering a rapid signal during a transient association, leading later to deletion.
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- T-CELL DIFFERENTIATION; MLSA-ENCODED ANTIGENS; NEONATAL TOLERANCE; DENDRITIC CELLS; DETERMINANTS; MACROPHAGES; COMPLEX; ANTIBODIES; INDUCTION
- Publisher's Version
- https://doi.org/10.1016/0165-2478(91)90004-T
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1991-06-01 12:00:00