Characterization of hematopoietic progenitor cells that express the transcription factor SCL, using a lacZ &quot;knock-in&quot; strategy

Elefanty, AG; Begley, CG; Metcalf, D; Barnett, L; Kontgen, F; Robb, L

Characterization of hematopoietic progenitor cells that express the transcription factor SCL, using a lacZ "knock-in" strategy

Author(s): Elefanty, AG; Begley, CG; Metcalf, D; Barnett, L; Kontgen, F; Robb, L;
Details: Publication Year 1998-09-29,Volume 95,Issue #20,Page 11897-11902
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. To study the functional properties of hematopoietic cells expressing SCL, we have generated mutant mice (SCLlacZ/w) in which the Escherichia coli lacZ reporter gene has been "knocked in" to the SCL locus, thereby linking beta-galactosidase expression to transcription from the SCL promoter. Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZ(high), lacZ(int), and lacZ(neg)). Cells that were lacZ(high) or lacZ(int) were enriched for day 12 spleen colony-forming units and myeloid and erythroid colony-forming cells (CFCs). These fractions included >99% of the erythroid and >90% of the myeloid CFCs, Culture of sorted bone marrow populations on stromal cells secreting interleukin-7 or in fetal thymic organ cultures showed that B and T lymphoid progenitors were also present in the lacZ(high) and lacZ(int) fractions. These data provide a functional correlation between SCL expression and colony-forming ability in immature hematopoietic cells. Our data also suggested that expression of SCL was transient and confined to hematopoietic stem and/or progenitor cells, because the differentiated progeny of most lineages (except the erythroid) were beta-galactosidase-negative.
Publisher: NATL ACAD SCIENCES
Keywords: STEM-CELLS; B-CELLS; GENE; DIFFERENTIATION; MICE; SCL/TAL-1; LINEAGES; INVITRO; ABSENCE; THYMUS
Publisher's Version: https://doi.org/10.1073/pnas.95.20.11897
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1998-09-29 12:00:00