Mice lacking the transcription factor CIITA - a second look
- Author(s)
- Williams, GS; Malin, M; Vremec, D; Chang, CH; Boyd, R; Benoist, C; Mathis, D;
- Details
- Publication Year 1998-12,Volume 10,Issue #12,Page 1957-1967
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- We have generated a second line of mice lacking a transcription factor thought to be a critical regulator of MHC class II gene expression, CIITA (for class II transactivator), Our and the previously published lines differ in the deletion that was engineered and by the fact that we removed the neomycin-resistance promoter and structural gene via the cre-IoxP recombination system. Characterization of our line led to two new findings. First, a substantial number of cells can express class II molecules in the absence of CIITA, albeit at 5-fold reduced levels, most notably dendritic cells in s.c. lymph nodes; therefore, the CIITA gene cannot be an absolute 'master gene' controlling the expression of class II molecules, as had been thought. Second, in contrast to recent results on human cell lines, CIITA is not critically involved in the IFN-gamma-induced up-regulation of MHC class I genes.
- Publisher
- OXFORD UNIV PRESS
- Keywords
- CLASS-II TRANSACTIVATOR; BARE LYMPHOCYTE SYNDROME; HLA CLASS-II; THYMIC EPITHELIAL-CELLS; CD4(+) T-CELLS; MONOCLONAL-ANTIBODIES; IN-VIVO; GLYCOPROTEIN FAMILY; DEFICIENCY SYNDROME; GENE-EXPRESSION
- Publisher's Version
- https://doi.org/10.1093/intimm/10.12.1957
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1998-12-01 12:00:00