Anterograde transport of leukemia inhibitory factor within transected sciatic nerves
- Author(s)
- Bennett, TM; Dowsing, BJ; Austin, L; Messina, A; Nicola, NA; Morrison, WA;
- Details
- Publication Year 1999-01,Volume 22,Issue #1,Page 78-87
- Journal Title
- MUSCLE & NERVE
- Publication Type
- Journal Article
- Abstract
- Disappointing functional recovery following peripheral nerve repair can be improved by neurotrophic growth factors. Leukemia inhibitory factor (LIF) is unique in that it has independent neurotrophic and myotrophic actions. The aim of this study was to explain this finding by establishing the existence of anterograde axonal transport of LIF from the site of nerve division to denervated muscles. Using I-125 LIF, administered topically via an entubulation repair of divided rat sciatic nerve, we monitored its subsequent distribution by measuring the radioactivity associated with nerve segments and denervated muscles. We established that LIF preferentially accumulated in denervated muscles, a process we could reduce by 70% after tightly ligating the intervening nerve, confirming the presence of anterograde axonal transport. This was most likely an active mode of transport that ceased approximately 24 h after nerve division, establishing a narrow clinical time frame within which the myotrophic action of LIF could be optimized following nerve repair. (C) 1999 John Wiley & Sons, Inc.
- Publisher
- JOHN WILEY & SONS INC
- Keywords
- AXONAL-TRANSPORT; MESSENGER-RNA; SIGNAL-TRANSDUCTION; SYMPATHETIC NEURONS; NEUROTROPHIC FACTOR; SKELETAL-MUSCLE; FACTOR-RECEPTOR; GROWTH-FACTOR; CELL-DEATH; RAT
- Publisher's Version
- https://doi.org/10.1002/(SICI)1097-4598(199901)22:1<78::AID-MUS12>3.0.CO;2-I
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- Refer to copyright notice on published article.
Creation Date: 1999-01-01 12:00:00