Pore formation by dimeric Bak and Bax: an unusual pore?
Author(s)
Uren, RT; Iyer, S; Kluck, RM;
Journal Title
Philos Trans R Soc Lond B Biol Sci
Publication Type
Journal Article
Abstract
Apoptotic cell death via the mitochondrial pathway occurs in all vertebrate cells and requires the formation of pores in the mitochondrial outer membrane. Two Bcl-2 protein family members, Bak and Bax, form these pores during apoptosis, and how they do so has been investigated for the last two decades. Many of the conformation changes that occur during their transition to pore-forming proteins have now been delineated. Notably, biochemical, biophysical and structural studies indicate that symmetric homodimers are the basic unit of pore formation. Each dimer contains an extended hydrophobic surface that lies on the outer membrane, and is anchored at either end by a transmembrane domain. Membrane-remodelling events such as positive membrane curvature have been reported to accompany apoptotic pore formation, suggesting Bak and Bax form lipidic pores rather than proteinaceous pores. However, it remains unclear how symmetric dimers assemble to porate the membrane. Here, we review how clusters of dimers and their lipid-mediated interactions provide a molecular explanation for the heterogeneous assemblies of Bak and Bax observed during apoptosis.This article is part of the themed issue 'Membrane pores: from structure and assembly, to medicine and technology'.
Publisher
Royal Society
WEHI Research Division(s)
Molecular Genetics Of Cancer
PubMed ID
28630157
NHMRC Grants
NHMRC/637337 NHMRC/1008434 NHMRC/1016701
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2017-06-26 02:15:32
Last Modified: 2018-10-15 09:36:04
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