DiSNE movie visualization and assessment of clonal kinetics reveal multiple trajectories of dendritic cell development
Details
Publication Year 2018-03-06, Volume 22, Issue #10, Page 2557-2566
Journal Title
Cell Reports
Publication Type
Journal Article
Abstract
A thorough understanding of cellular development is incumbent on assessing the complexities of fate and kinetics of individual clones within a population. Here, we develop a system for robust periodical assessment of lineage outputs of thousands of transient clones and establishment of bona fide cellular trajectories. We appraise the development of dendritic cells (DCs) in fms-like tyrosine kinase 3 ligand culture from barcode-labeled hematopoietic stem and progenitor cells (HSPCs) by serially measuring barcode signatures and visualize these multidimensional data using developmental interpolated t-distributed stochastic neighborhood embedding (DiSNE) time-lapse movies. We identify multiple cellular trajectories of DC development that are characterized by distinct fate bias and expansion kinetics and determine that these are intrinsically programmed. We demonstrate that conventional DC and plasmacytoid DC trajectories are largely separated already at the HSPC stage. This framework allows systematic evaluation of clonal dynamics and can be applied to other steady-state or perturbed developmental systems.
Publisher
Cell Press
WEHI Research Division(s)
Immunology; Molecular Medicine
PubMed ID
29514085
NHMRC Grants
NHMRC/1062820 NHMRC/1100033 NHMRC/1145184
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2018-03-19 10:02:37
Last Modified: 2018-03-19 11:53:01
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