Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing
Details
Publication Year 2021-11-11, Volume 22, Issue #1, Page 310
Journal Title
Genome Biology
Abstract
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation detection in single cells. We identify thousands of unannotated isoforms and find conserved functional modules that are enriched for alternative transcript usage in different cell types and species, including ribosome biogenesis and mRNA splicing. Analysis at the transcript level allows data integration with scATAC-seq on individual promoters, improved correlation with protein expression data, and linked mutations known to confer drug resistance to transcriptome heterogeneity.
Publisher
BMC
Keywords
Long-read sequencing; Single-cell gene expression; Single-cell multi-omics; Splicing
WEHI Research Division(s)
Epigenetics And Development; Blood Cells And Blood Cancer; Advanced Technology And Biology
PubMed ID
34763716
Open Access at Publisher's Site
https://doi.org/10.1186/s13059-021-02525-6
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2021-12-07 12:04:16
Last Modified: 2021-12-09 02:06:29
An error has occurred. This application may no longer respond until reloaded. Reload 🗙